A Huge Diversity of Metopids (Ciliophora, Armophorea) in Soil from the Murray River Floodplain, Australia. II. Morphology and Morphogenesis of Lepidometopus platycephalus nov. gen., nov. spec.

Peter Vďačný,

Wilhelm Foissner

Abstrakt

The morphology and morphogenesis of a new Australian metopid ciliate, Lepidometopus platycephalus nov. gen., nov. spec., were studied using live observation, various silver impregnation methods, scanning electron microscopy, and morphometry. The new genus is outstanding in having epicortical scales (lepidosomes) and a strongly flattened and distinctly projecting preoral dome. Diagnostic features of L. platycephalus include a small, reniform body carrying an elongated caudal cilium, about 11 ciliary rows, and an adoral zone composed of an average of 11 polykinetids. The morphogenesis of L. platycephalus matches data from other metopids in that (1) the body is drastically re-shaped, (2) the parental oral structures are reorganized but do not contribute to the daughter oral ciliature, (3) the opisthe’s adoral polykinetids originate pleurotelokinetally, (4) the opisthe’s paroral membrane is formed via re-arrangement of the posterior portion of the first two perizonal rows, and (5) the opisthe’s perizonal stripe is made by three parental perizonal rows and two dorsolateral ciliary rows. The morphogenetic data corroborate phylogenetic analyses in that caenomorphids are only superficially similar to metopids; metopids and clevelandellids are closely related; and litostomateans are the best candidates for a sister group of the metopid-clevelandellid assemblage within the SAL superclade.

Słowa kluczowe: Binary fission, lepidosomes, Metopidae, morphometry, stomatogenesis
References

Aescht E. (2001) Catalogue of the generic names of ciliates (Protozoa, Ciliophora). Denisia 1: 1–350

Bourland W. A., Wendell L. (2014) Redescription of Atopospira galeata (Kahl, 1927) nov. comb. and A. violacea (Kahl, 1926) nov. comb. with redefinition of Atopospira Jankowski, 1964 nov. stat. and Brachonella Jankowski, 1964 (Ciliophora, Armophorida). Eur. J. Protistol. 50: 356–372

Bourland W. A., Wendell L., Hampikian G. (2014) Morphologic and molecular description of Metopus fuscus Kahl from North America and new rDNA sequences from several metopids (Armophorea, Metopidae). Eur. J. Protistol. 50: 213–230

Bourland W., Rotterová J., Čepička I. (2017) Redescription and molecular phylogeny of the type species for two main metopid genera, Metopus es (Müller, 1776) Lauterborn, 1916 and Brachonella contorta (Levander, 1894) Jankowski, 1964 (Metopida,Ciliophora), based on broad geographic sampling. Eur. J. Protistol. 59: 133–154

Claparède E., Lachmann J. (1858) Études sur les infusoires et les rhizopodes. Mém. Inst. Natn. Génev. 5: 1–260

da Silva-Neto I. D., Paiva T. da S., do Nascimento Borges B., Harada M. L. (2015) Fine structure and molecular phylogeny of Parametopidium circumlabens (Ciliophora: Armophorea), endocommensal of sea urchins. J. Eukaryot. Microbiol. 63: 46–51

Esteban G., Fenchel T., Finlay B. (1995) Diversity of free-living morphospecies in the ciliate genus MetopusArch. Protistenkd. 146: 137–164

Foissner W. (1991) Basic light and scanning electron microscopic methods for taxonomic studies of ciliated protozoa. Eur. J. Protistol. 27: 313–330

Foissner W. (1996) Ontogenesis in ciliated protozoa, with emphasis on stomatogenesis. In: Ciliates: Cells as Organisms, (Eds. K. Hausmann, B. C. Bradbury). Fischer Verlag, Stuttgart, Jena, New York, 95–177

Foissner W. (1998) An updated compilation of world soil ciliates (Protozoa, Ciliophora), with ecological notes, new records, and descriptions of new species. Eur. J. Protistol. 34: 195–235

Foissner W. (2005a) Two new “flagship” ciliates (Protozoa, Ciliophora) from Venezuela: Sleighophrys pustulata and Luporinophrys micelaeEur. J. Protistol. 41: 99–117

Foissner W. (2005b) The unusual, lepidosome-coated resting cyst of Meseres corlissi (Ciliophora: Oligotrichea): transmission electron microscopy and phylogeny. Acta Protozool. 44: 217–230

Foissner W. (2014) An update of ‘basic light and scanning electron microscopic methods for taxonomic studies of ciliated protozoa’. Int. J. Syst. Evol. Microbiol. 64: 271–292

Foissner W. (2016a) Protists as bioindicators in activated sludge: identification, ecology and future needs. Eur. J. Protisol. 55: 75–94

Foissner W. (2016b) Terrestrial and semiterrestrial ciliates (Protozoa, Ciliophora) from Venezuela and Galápagos. Denisia 35: 1–912

Foissner W. (2016c) Heterometopus meisterfeldi nov. gen., nov. spec. (Protozoa, Ciliophora), a new metopid from Australia. Eur. J. Protistol. 55: 118–127

Foissner W., Agatha S. (1999) Morphology and morphogenesis of Metopus hasei Sondheim, 1929 and M. inversus (Jankowski, 1964) nov. comb. (Ciliophora, Metopida). J. Eukaryot. Microbiol. 46: 174–193

Foissner W., Xu K. (2007) Monograph of the Spathidiida (Ciliophora, Haptoria). Vol. I: Protospathidiidae, Arcuospathidiidae, Apertospathulidae. Monograph. Biol. 81: 1–485

Foissner W., Berger H., Kohmann F. (1992) Taxonomische und ökologische Revision der Ciliaten des Saprobiensystems – Band II: Peritrichia, Heterotrichida, Odontostomatida. Informationsberichte des Bayerischen Landesamtes für Wasserwirtschaft 5/92: 1–502

Foissner W., Agatha S., Berger H. (2002) Soil ciliates (Protozoa, Ciliophora) from Namibia (Southwest Africa), with emphasis on two contrasting environments, the Etosha region and the Namib Desert. Denisia 5: 1–1459

Foissner W., Müller H., Agatha S. (2007) A comparative fine structural and phylogenetic analysis of resting cysts in oligotrich and hypotrich Spirotrichea (Ciliophora). Eur. J. Protistol. 43: 295–314

Gaievskaïa N. (1925) Sur deux nouveaux infusoires des mares salées – Cladotricha koltzowii nov. gen. nov. sp. et Palmarium salinum nov. gen. nov. sp. Russk. Arkh. Protist. 4: 255–288

Gao F., Warren A., Zhang Q., Gong J., Miao M., Sun P., Xu D., Huang J., Yi Z., Song W. (2016) The all-data-based evolutionary hypothesis of ciliated protists with a revised classification of the phylum Ciliophora (Eukaryota, Alveolata). Sci. Rep. 6: 24874

Gentekaki E., Kolisko M., Boscaro V., Bright K. J., Dini F., Di Giuseppe G., Gong Y., Miceli C., Modeo L., Molestina R. E., Petroni G., Pucciarelli S., Roger A. J., Strom S. L., Lynn D. H. (2014) Large-scale phylogenomic analysis reveals the phylogenetic position of the problematic taxon Protocruzia and unravels the deep phylogenetic affinities of the ciliate lineages. Mol. Phylogenet. Evol. 78: 36–42

Gentekaki E., Kolisko M., Gong Y., Lynn D. (2017) Phylogenomics solves a long-standing evolutionary puzzle in the ciliate world: The subclass Peritrichia is monophyletic. Mol. Phylogenet. Evol. 106: 1–5

Hu X. (2014) Ciliates in extreme environments. J. Eukaryot. Microbiol. 61: 410–418

International Commission on Zoological Nomenclature [ICZN] (1999) International Code of Zoological Nomenclature. 4th ed. Tipografia La Garangola, Padova

Jankowski A. W. (1964a) Morphology and evolution of Ciliophora. III. Diagnoses and phylogenesis of 53 sapropelebionts, mainly of the order Heterotrichida. Arch. Protistenkd. 107: 185–294

Jankowski A. W. (1964b) Morfologiâ i èvolûciâ Ciliophora. I. Novaâ sistema sapropelebiotičeskih Heterotrichida [Morphology and evolution of Ciliophora. I. The new system of sapropelebiotic Heterotrichida]. Zool. Zh. 43: 503–517 (in Russian with English title translation and summary)

Jankowski A. W. (1975) Konspekt novoj sistemy podtipa Ciliophora Doflein, 1901 [A conspectus of the new system of the subphylum Ciliophora Doflein, 1901]. In: Account of scientific sessions on results of scientific work: Abstracts of reports, (Ed. U. S. Balašov). Akademiâ Nauk SSSR, Zool. Inst., Leningrad, 26–27 (in Russian)

Jankowski A. W. (1980) Konspekt novoj sistemy tipa Ciliophora [Conspectus of a new system of the phylum Ciliophora]. Trudy Zool. Inst. (Leningr.) 94: 103–121 (in Russian)

Jankowski A. W. (2007) Tip Ciliophora Doflein, 1901 [Phylum Ciliophora Doflein, 1901]. In: Protisty: Rukovodstvo po zoologii, č. 2 [Protista: Handbook of Zoology, 2nd part], (Ed. A. F. Alimov). Nauka, St. Petersburg, 415–993 (in Russian with English title translation)

Kahl A. (1927) Neue und ergänzende Beobachtungen heterotricher Ciliaten. I. Arch. Protistenkd. 57: 121–203

Kahl A. (1930) Urtiere oder Protozoa I: Wimpertiere oder Ciliata (Infusoria) 1. Allgemeiner Teil und Prostomata. Tierwelt Dtl. 18: 1–180

Kahl A. (1932) Urtiere oder Protozoa I: Wimpertiere oder Ciliata (Infusoria) 3. Spirotricha. Tierwelt Dtl. 25: 399–650

Levander K. M. (1894) Materialien zur Kenntniss der Wasserfauna in der Umgebung von Helsingfors, mit besonderer Berücksichtigung der Meeresfauna. I. Protozoa. Acta Soc. Fauna Flora Fenn. 12: 1–115

Li M., Sun Z.-Y., Grim J. N., Ponce-Gordo F., Wang G.-T., Zou H., Li W.-X., Wu S.-G. (2016) Morphology of Nyctotheroides hubeiensis Li et al. 1998 from frog hosts with molecular phylogenetic study of clevelandellid ciliates (Armophorea, Clevelandellida). J. Eukaryot. Microbiol. 63: 751–759

Li M., Li C., Grim J. N., Ponce-Gordo F., Wang G., Zou H., Li W., Wu S. (2017) Supplemental description of Nyctotheroides pyriformis n. comb. (= Macrocytopharynxa pyriformis (Nie, 1932) Li et al. 2002) from frog hosts with consideration of the validity of the genus Macrocytopharynxa (Armophorea, Clevelandellida). Eur. J. Protistol. 58: 152–163

Lynn D. H. (2004) Morphology or molecules: How do we identify the major lineages of ciliates (Phylum Ciliophora)? Eur. J. Protistol. 39: 356–364

Lynn D. H. (2008) The Ciliated Protozoa. Characterization, Classification and Guide to the Literature. 3rd ed. Springer, Dordrecht

Lynn D. H., Wright A.-D. G. (2013) Biodiversity and molecular phylogeny of Australian Clevelandella species (class Armophorea, order Clevelandellida, family Clevelandellidae), intestinal endosymbiotic ciliates in the wood-feeding roach Panesthia cribrata Saussure, 1864. J. Eukaryot. Microbiol. 60: 335–341

Martín-González A., Serrano S., Fernández-Galiano D. (1987) Cortical morphogenesis and conjugation process in Caenomorpha medusula (Ciliophora, Heterotrichida). Eur. J. Protistol. 23: 111–121

Nicholls K. H., Lynn D. H. (1984) Lepidotrachelophyllum fornicis, n. g., n. sp., a ciliate with an external layer of organic scales (Ciliophora, Litostomatea, Haptoria). J. Protozool. 31: 413–419

Omar A., Zhang Q., Zou S., Gong J. (2017) Morphology and phylogeny of the soil ciliate Metopus yantaiensis n. sp. (Ciliophora, Metopida), with identification of the intracellular bacteria. J. Eukaryot. Microbiol. (in press) doi:10.1111/jeu.12411

Paiva T. da S., do Nascimento Borges B., Silva-Neto I. D. (2013) Phylogenetic study of class Armophorea (Alveolata, Ciliophora) based on 18S-rDNA data. Genet. Mol. Biol. 36: 571–585

Perty M. (1849) Mikroskopische Organismen der Alpen und der italienischen Schweiz. Mitt. Naturf. Ges. Bern 1849: 153–176

Poche F. (1913) Das System der Protozoa. Arch. Protistenkd. 30: 125–321

Puytorac P. de (1994) Phylum Ciliophora Doflein, 1901. In: Traité de zoologie, infusoires ciliés, Vol. 2, (Ed. P. de Puytorac). Masson, Paris, 1–15

Puytorac P. de, Grain, J. (1976). Ultrastructure du cortex buccal et évolution chez les ciliés. Protistologica 12: 49–67

Saccà A. (2012) The role of eukaryotes in the anaerobic food web of stratified lakes. In: Anoxia: Evidence for Eukaryote Survival and Paleontological Strategies, Cellular Origin, Life in Extreme Habitats and Astrobiology, (Eds. A. Altenbach, J. M. Bernhard, J. Seckbach). Springer, Dordrecht, Heidelberg, London, New York, 403–419

Santos S. M., Guinea A., Fernández-Galiano D. (1986) Étude de l’infraciliature et du processus de bipartition chez Nyctotherus ovalis Leidy, 1850 (Ciliophora, Heterotrichida). Protistologica 22: 351–358

Small E. B., Lynn D. H. (1985) Phylum Ciliophora Doflein, 1901. In: An Illustrated Guide to the Protozoa, (Eds. J. J. Lee, S. H. Hutner, E. C. Bovee). Society of Protozoologists, Allen Press, Lawrence, Kansas, 393–575

Tirjaková E., Krajčovičová K., Illyová M., Vďačný P. (2016) Interaction of ciliate communities with cyanobacterial water bloom in a shallow, hypertrophic reservoir. Acta Protozool. 55: 173–188

Vďačný P., Foissner W. (2012) Monograph of the dileptids (Protista, Ciliophora, Rhynchostomatia). Denisia 31: 1–529

Vďačný P., Foissner W. (2017) A huge diversity of metopids (Ciliophora, Armophorea) in soil from the Murray River floodplain, Australia. I. Description of five new species and redescription of Metopus setosus Kahl, 1927. Eur. J. Protistol. 57: 35–76

Vďačný P., Orsi W., Foissner W. 2010. Molecular and morphological evidence for a sister group relationship of the classes Armophorea and Litostomatea (Ciliophora, Intramacronucleata, Lamellicorticata infraphyl. nov.), with an account on basal haptorid litostomateans. Eur. J. Protistol. 46: 298–309

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