Taxonomic and Morphogenetic Description of the Freshwater Ciliate Aponotohymena isoaustralis n. sp. (Ciliophora; Oxytrichidae) Isolated from Sanjay Lake, Delhi, India

Renu Gupta,

Jeeva Susan Abraham,

Sripoorna Somasundaram,

Ravi Toteja,

Seema Makhija,

Hamed A. El-Serehy

Morphology, morphogenesis and molecular phylogeny of a freshwater oxytrichid ciliate, Aponotohymena isoaustralis n. sp. collected from Sanjay Lake (28°36′51″N, 77°18′14″E), Delhi, India, were studied. The described species is characterized by a flexible body, with body size (in vivo) of about 148 × 46 µm and yellowish green cortical granules. Morphological characters exhibit: undulating membranes in Notohymena–pattern; two macronuclei and absence of micronucleus (amicronucleate); about 36 adoral membranelles; 18 frontoventral-transverse (FVT) cirri; one right and one left marginal row separated posteriorly; 6 dorsal rows; 7 caudal cirri arranged in 2 + 2 + 3 pattern (constant). In the present study, a detailed description of all the developmental stages is also provided. Prominent distinguishing features of the new species are the absence of micronucleus, 7 caudal cirri (constant), yellowish green cortical granules aligned along the margins and irregularly distributed throughout the cell. They may also be randomly concentrated as clusters along the left margin and posterior end of the cell. Molecular phylogeny based on small subunit rDNA sequence data suggests sister relationship of Aponotohymena isoaustralis n. sp. with Notohymena apoaustralis and Aponotohymena australis (Notohymena australis) which cluster in a clade with Paraurostyla weissei and Paraurostyla coronata. Further analysis of nucleotide sequence of SSU rDNA also suggests that A. isoaustralis n. sp. is distinct from the type species A. australis.
Słowa kluczowe: Aponotohymena, morphogenesis, morphology, oxytrichid, phylogeny, SSU rDNA

Arora S., Gupta R., Kamra K., Sapra G. R. (1999) Characterization of Paraurostyla coronata sp. n. including a comparative account of other members of the genus. Acta Protozool. 38: 133–144

Berger H., Foissner W. (1997) Cladistic relationships and generic characterization of oxytrichid hypotrichs (Protozoa, Ciliophora). Arch. Protistenk. 148: 125–155

Berger H. (1999) Monograph of Oxytrichidae (Ciliophora, Hypotrichia). Monographiae Biologicae. Springer 78: 1–1080

Berger H. (2008) Monograph of Amphisiellidae and Trachelostylidae (Ciliophora, Hypotricha). Monographiae Biologicae. Springer 88: 1–737

Berger H. (2011) Monograph of the Gonostomatidae and Kahliellidae (Ciliophora, Hypotricha) (Monographiae Biologicae, vol. 90). Springer 90: 1–742

Berger H., Foissner W. (1997) Cladistic relationships and generic characterization of oxytrichid hypotrichs (Protozoa, Ciliophora). Arch. Protistenkd148: 125–155

Blatterer H., Foissner W. (1988) Beitrag zur terricolen Ciliatenfauna (Protozoa: Ciliophora) Australiens. Stapfia.17: 1–84

Borror A. C. (1972) Revision of the order Hypotrichida (Ciliophora, Protozoa). J. Protozool. 19: 1–23

Chapman–Andresen C. (1958) Pinocytosis of inorganic salts by Amoeba proteus (Chaos diffluens). C. R. Trav. Lab. Carlsberg. Chim. 31: 77–92

Chen X., Li L., Hu X., Shao C., Al-Farraj S. A., Al-Rasheid K. A. S. (2013) A morphogenetic description of Thigmokeronopsis stoecki Shao et al. 2008 (Ciliophora, Hypotricha) and a comparison with members of the family Pseudokeronopsidae. Acta Protozool52: 65–72

Chieco P., Derenzini M. (1999) The feulgen reaction 75 years on. Histochem. Cell. Biol. 111: 345–358

Doerder F. P. (2014) Abandoning sex: multiple origins of asexuality in the ciliate Tetrahymena. BMC evol. Biol. 14: 112

Ehrenberg C. G. (1830) Beiträge zur Kenntnniß der Organisation der Infusorien und ihrer geographischen Verbreitung, besonders in Sibirien. Abh. Preuss. Akad. Wiss., Phys. Math. K. I. 1–88

Fauré-Fremiet E. (1961) Remarques sur la morphologie comparée et la systématique des ciliata Hypotrichida. C. r. hebd. Séanc. Acad. Sci., Paris252: 3515–3519

Foissner W. (1983) Die Morphogenese von Urosoma macrostyla (Wrzésniowski 1870) (Ciliophora: Oxytrichidae). Arch. Protistenkd. 127: 413–428

Foissner W., Adam H. (1983a) Die Morphogenese von Urosomoida agiliformis Foissner 1982 (Ciliophora, Oxytrichidae). Zool. Anz. 211: 161–176

Foissner W., Adam H. (1983b) Morphologie and morphogenese des Bodenciliaten Oxytricha granulifera sp. n. (Ciliophora, Oxytrichidae). Zool. Scr. 12: 1–11

Foissner W., O’ Donoghue P. J. (1990) Morphology and infraciliature of some freshwater ciliates (Protozoa: Ciliophora) from Western and South Australia. Invertebr. Taxon. 3: 661–696

Foissner W. (1996) Faunistics, taxonomy and ecology of moss and soil ciliates (Protozoa, Ciliophora) from Antarctica, with description of new species, including Pleuroplitoides smithi gen. n., sp. n. Acta Protozool. 35: 95–123

Foissner W., Stoeck T. (2011) Cotterillia bromelicola nov. gen., nov. spec., a gonostomatid ciliate (Ciliophora, Hypotricha) from tank bromeliads (Bromeliaceae) with de novo originating dorsal kineties. Eur. J. Protistol. 47: 29–50

Foissner W. (2016) Terristrial and semi-terristrial ciliates (Protozoa, Ciliophora) from Venezuela and Galápagos. Denisia 35: 1–912

Ganner B., Foissner W., Adam H. (1986) Morphogenetic and biometric comparison of four populations of Urosomoida agiliformis (Ciliophora, Hypotrichida). Annls Sci. Nat. (13e Series) 8: 199–207

Gupta R., Kamra K., Sapra G. R. (2006) Morphology and cell division of the oxytrichids Architricha indica nov. gen., nov. sp., and Histriculus histrio (Müller, 1773), Corliss, 1960 (Ciliophora, Hypotrichida). Eur. J. Protistol. 42: 29–48

Hemberger H. (1985) Neue Gattungen und Arten hypotricher Ciliaten. Arch. Protistenk. 130: 397–417

Hall T. A. (1999) BioEdit: a user friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl. Acids Symp. Ser. 41: 95–98

Hu X., Kusuoka Y. (2015) Two oxytrichids from ancient lake Biwa, Japan, with notes on morphogenesis of Notohymena australis (Ciliophora, Sporadotrichida). Acta Protozool. 54: 107–122

Kamra K., Sapra G. R. (1990) Partial retention of parental ciliature during morphogenesis of the ciliate Coniculostomum monilata (Dragesco and Njiné 1971) Njiné 1978 (Oxytrichidae, Hypotrichida). Eur. J. Protistol. 25: 264–278

Kamra K., Kumar S. (2010) Notohymena saprai sp. nov, a new oxytrichid ciliate (Protozoa, Ciliophora) from the valley of flowers, a Himalayan bioreserve region; description and morphogenesis of the new species. Indian J. Microbiol. 50: 33–45

Küppers G. C., Claps M. C., Lopretto E. C. (2007) Description of Notohymena pampasica n. sp. (Ciliophora, Stichotrichia). Acta Protozool. 46: 221–227

Küppers G. C., Paiva T. da S., Borges B. do N., Harada M. L., Garraza G. G., Mataloni G. (2011) An Antartic hypotrichous ciliate, Parasterkiella thompsoni (Foissner) nov. gen., nov. comb., recorded in Argentinean peat–bogs: morphology, morphogenesis and molecular phylogeny. Eur. J. Protistol. 47: 103–123

Larkin M. A., Blackshields G., Brown N. P., Chenna R., McGettigan P. A., McWilliam H., Valentin F., Wallace I. M., Wilm A., Lopez R., Thompson J. D., Gibson T. J., Hiqqins D. G. (2007) Clustal W and Clustal X version 2.0. Bioinformatics 23: 2947–2948

Li F., Lv Z., Yi Z., Al-Farraj S. A., Al-Rasheid K. A. S., Shao C. (2014) Taxonomy and phylogeny of two species of the genus Deviata (Protista, Ciliophora) from China,with description of a new soil form, Deviata parabacilliformis sp. nov. Int. J. Syst. Evol. Microbiol. 64: 3775–3785

Lv Z., Chen L., Chen L., Shao C., Miao M., Warren A. (2013) Morphogenesis and molecular phylogeny of a new freshwater ciliate, Notohymena apoaustralis n. sp. (Ciliophora: Oxytrichidae). J. Eukaryot. Microbiol. 60: 455–466

Medlin L., Elwood H. J., Stickel S., Sogin M. L. (1988) The characterization of enzymatically amplified eukaryotic 16 S–like rRNA– coding regions. Gene 71: 491–499

Naqvi I., Gupta R., Borgohain P., Sapra G. R. (2006) Morphology and morphogenesis of Rubrioxytricha indica n. sp. (Ciliophora: Hypotrichida). Acta Protozool. 45: 53–64

Naqvi I., Gupta R., Makhija S.Toteja R., Abraham J. S., Somasundaram S., El-Serehy H. A. (2016) Morphology and morphogenesis of a new oxytrichid ciliate, Notohymena limus n. sp. (Ciliophora, Oxytrichidae) from Delhi, India. Saudi J. Biol. Sci. 23: 789–794

Prescott D. M. (1994) The DNA of ciliated protozoa. Microbiol. Rev. 58: 233–267

Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. (2011) MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance and maximum parsimony methods. Mol. Biol. Evol. 28: 2731–2739

Voss H. J. (1991a) Die Morphogenese von Cyrtohymena muscorum (Kahl, 1932) Foissner, 1989 (Ciliophora, Oxytrichidae). Arch. Protistenkd. 140: 67–81

Voss H. J. (1991b) Die Morphogenese von Notohymena rubescens Blatterer and Foissner 1988 (Ciliophora, Oxytrichidae). Arch. Protistenkd. 140: 219–236

Voss H. J., Foissner W. (1996) Divisional morphogenesis in Steinia sphagnicola (Ciliophora, Hypotrichida): a comparative light and scanning electron microscope study. Europ. J. Protistol. 32: 31–46

Voss H. J. (2008) Divisional morphogenesis of Notohymena australis (Foissner and O’ Donoghue, 1990) Berger 1999 (Ciliophora, Hypotrichida, Oxytrichidae). Denisia 23: 289–295

Wallengren H. (1900) ZurKenntnis der vergleichenden Morphologie der hypotrichen Infusorien. Bih. K. Svensk Vetensk Akad. Handl. 26: 1–31

Wilbert N. (1975) Eine verbesserte Technik der Protargol imprägnation für Ciliaten. Mikrokosmos 64: 171–179

Wirnsberger E., Foissner W., Adam H. (1985) Morphological, biometric and morphogenetic comparison of two closely related species, Stylonychia vorax and S. pustulata (Ciliophora: Oxytrichidae). J. Protozool. 32: 261–268

Wirnsberger E., Foissner W., Adam H. (1986) Biometric and morphogenetic comparison of the sibling species Stylonychia mytilus and S. lemnae, including a phylogenetic system for the oxytrichids (Ciliophora: Hypotrichida). Arch. Protistenkd132: 167 –185

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