Actinomycin D as an Epimutagen in Protists

Zoya I. Uspenskaya,

Alexander L. Yudin

Abstrakt

A previously unrecorded property of actinomycin D, a well-known antibiotic, was discovered in the course of long-term genetic research on the amoeba Amoeba proteus and the ciliate Dileptus anser. In these protists actinomycin D can induce an unusual type of hereditary variation, which we refer to as the inheritable destabilization of characters. A number of features indicate that this variation is epigenetic, that is, not caused by mutations in the DNA. Therefore, actinomycin D may be considered as an inducer of epigenetic inheritable changes, in other words, as an epimutagen.

 

Słowa kluczowe: actinomycin D, Amoeba proteus, Dileptus anser, epigenetic variation, epimutagen, inheritable destabilization of characters
References

Adl S. M., Simpson A. G. B., Lane C. E., Lukeš J., Bass D. et al. (2012) The revised classification of eukaryotes. J. Eukaryot. Microbiol59: 429–493

Arai Y., Hayakawa K., Arai D., Ito R., Iwasaki Y. et al. (2015) Putative epimutagens in maternal peripheral and cord blood samples identified using human induced pluripotent stem cells. BioMed. Res. Int. Article ID 876047, doi: 10.1155/2015/876047

Beale G. H., Preer J. R. (2008) Paramecium: Genetics and Epigenetics. CRC Press. Taylor and Francis Group, London, New York

Bleyman L. K. (1971) Temporal patterns in the ciliated protozoa. In: Developmental aspects of the cell cycle, Academic Press, New York, 67–91

Bleyman L. K. (1972) A new spontaneous early mature mutant in Tetrahymena pyriformisGenetics 71: s5–s6

Cervantes M. D., Hamilton E. P., Xiong J., Lawson M. J., Yuan D., Hadjithomas M., Miao W., Orias E. (2013) Selecting one of several mating types through gene segment joining and deletion in Tetrahymena thermophilaPLoS Biol. 11: e1001518, doi: 10.1371/journal.pbio.1001518

Duharcourt S., de Lepère G., Meyer E. (2009) Developmental genome rearrangements in ciliates: a natural genomic subtraction mediated by non-coding transcripts. Trends Genet. 25 (8): 344–350

Fishbein L. (1979) Potential industrial carcinogens and mutagens. Elsevier Scientific Publishing Company, Amsterdam, Oxford, New York

Fishbein L., Flamm W. G., Falk H. L. (1970) Chemical mutagens. Academie Press, New York, London

Golubovsky M. D. (1996) Epigenes concept 20 years later. Biopolymers Cell 12: 5–24 (in Russia)

Holliday R., Ho T. (2002) DNA methylation and epigenetic inheritance. Methods 27: 179–183

Hollstein U. (1974) Actinomycin. Chemistry and mechanism of action. Chem. Rev. 74 (6): 625–652, doi:10.1021/cr60292a002

Jacob F., Monod J. (1963) Genetic repression, allosteric inhibition, and cellular differentiation. In: Cytodifferentiaton and macromolecular synthesis. New York, London, Academie Press, 30–64

Judd E. M., Laub M. T., McAdams H. H. (2000) Toggles and oscillators: new genetic circuit designs. BioEssays 22: 507–509

Kalinina L. V. (1967) Hereditary changes caused by x-irradiation in Amoeba proteusTsitologiya 9: 1543–1549 (in Russian with English summary)

Kalinina L. V. (1968) Hereditary changes in amoebae caused by actinomycin D treatment. Tsitologiya 10: 1589–1597 (in Russian with English summary)

Kalinina L. V. (1969) A study of the resistance to streptomycin in amoebae. Tsitologiya 11: 760–767 (in Russian with English summary)

Kalinina L. V., Yudin A. L. (1964) Genetic interacnion of nuclei in heterokaryons of amebas. Tsitologiya 6: 695–709 (in Russian with English summary)

Kataoka K., Mochizuki K. (2011) Programmed DNA elimination in Tetrahymena: a small RNA-mediated genome surveillance mechanism. Adv. Exp. Med. Biol. 722: 156–173

Koba M., Konopa J. (2005) Actinomycin D and its mechanisms of action. Postep. Hig. Med. Dosw. 59: 290–298 (in Polish with English summary)

Lamparska K., Clark J., Babilonia G., Bedell V., Yip W., Smith S.S. (2012) 2’-Deoxyriboguanylurea, the primary breakdown product of 5-aza-2’-deoxyribocytidine, is a mutagen, an epimutagen, an inhibitor of DNA methyltransferases and an inducer of 5-azacytidine-type fragile sites. Nucleic Acids Res40: 9788–801, doi: 10.1093/nar/gks706

Maletskaia E. I., Iudanova S. S., Maletskiĭ S. I. (2006) Effect of the epimutagen 5-azacytidine on the structure of floral-stalk metameres in sugar beet (Beta vulgaris L) Genetika 42: 939–946 (in Russian with English summary)

Meyer F., Chalker D. L. (2007) Epigenetics of Ciliates. Epigenetics, (Eds. C.D. Allis et al.) CSHL Press, 127–150

Miyake A. (1996) Fertilization and sexuality in Ciliates. In: Ciliates: Cells as organisms (eds. R. Hausmann and P.C. Bradbury), Stuttgard, Jena, New York, Gustav Fischer Verlag, 243–290

Monod J., Jacob F. (1961) General conclusions: teleonomic mechanisms in cellular metabolism, growth, and differentiation. Cold Spring Harbor Symp. Quant. Biol26: 389–401

Nanney D. L. (1958) Epigenetic control systems. Proc. Nat. Acad. Sci. U.S.A. 44: 712–716

Nanney D. L. (1980) Experimental Ciliatology. An Introduction to Genetics and Developmental Analysis in Ciliates (eds. John Wiley and Sons), New York, Chichester, Brisbane, Toronto

Nowacki M., Landweber L. (2009) Epigenetic inheritance in ciliates. Curr. Opin. Microbiol. 12: 638–643

Olenov J. M. (1970) Epigenomic variability. Ontogenez 1: 10–16 (in Russian with English summary)

Siegel R. (1970) Genetics of mating types in ciliates. Ontogenez 1: 157–165

Sobell H. (1985) Actinomycin and DNA transcription. Proc. Nat. Acad. Sci. U.S.A. 82: 5328–5331, doi:10.1073/pnas.82.16.5328

Takagi Y. (1988) Aging. In: Paramecium. Springer-Verlag, Berlin, 131–140

Tchuraev R. N. (1975) The epigene hypothesis. In: Studies on mathematical genetics, Issue of Scientific Papers (ed. Ratner V.A.). Novosibirsk, 77–94 (in Russian with English summary)

Tchuraev R. N. (2010) Epigenes – overgenes level hereditary units. Ecological Genetics 8: 17–24

Tchuraev R. N., Stupak I. V., Tropynina T. S., Srupak E. E. (2000) Epigenes: design and contrruction of new hereditary units. FEBS Lett. 486: 200–202

Uspenskaya Z. I., Yudin A. L. (2003) Mating types in the ciliate Dileptus anser. Genetical instability in the mating type system. Tsitologiya 45: 510–514 (in Russian with English summary)

Yudin A. L. (1967) Nuclear-nuclear interactions in amoeba. In: The control of nuclear activity. Englewood Cliffs, New Jersey, Prentice Hall, Inc., 299–318

Yudin A. L. (1973) Nuclear-nuclear interactions in heterokaryons. In: The biology of Amoeba, Academic Press, New York, London, 505–524

Yudin A. L. (1979а) Mechanisms of destabilization of hereditary characters in amoeba. II. Heritable variations induced by some antibiotics. Acta Protozool. 18: 571–580

Yudin A. L. (1979b) Nuclear transplantation studies in Amoeba proteus. Int. Rev. Cytol. Suppl. 9: 63–100

Yudin A. L. (1982) Nucleocytoplasmic relationships and cell heredity in amoebae. Leningrad, Nauka (in Russian)

Yudin A. L., Afonkin S.Yu. (1987) Genetic determination and inheritance of mating types in Dileptus anser (Gymnostomatida, Holotrricha). In: Current Problems of Protozoology. Nauka, Leningrad, 32 (in Russian)

Yudin A. L., Afonkin S. Yu., Parfenova E. V. (1990) Mating pheromones in the ciliate Dileptus anser. Tsitologiya 32: 107–116 (in Russian with English summary)

Yudin A. L., Sopina V. A. (1970) On the role of nucleus and cytoplasm in the inheritance of some characters in Amoebae (nuclear transfer experiments). Acta Protozool8: 1–39

Yudin A. L., Uspenskaya Z. I. (2006) Mating types in the ciliate Dileptus anser. Inheritance and genetic determination. Tsitologiya 48: 364–374 (in Russian with English summary)

Yudin A. L., Uspenskaya Z. I. (2007) Nuclear differentiation for mating types in the ciliate Dileptus anser. A hypothesis. Cell Biol. Int. 31: 428–432

Yudin A. L., Uspenskaya Z. I. (2009) Change of mating type induced by actinomycin D in the ciliate Dileptus anserTsitologiya 51: 84–88 (in Russian with English summary)

Czasopismo w darmowym dostępie.

Czasopismo ukazuje się w sposób ciągły on-line.
Pierwotną formą czasopisma jest wersja elektroniczna.