Taxonomical reinvestigation of the colepid species Pinacocoleps pulcher (Spiegel, 1926) Foissner et al., 2008 (Ciliophora: Prorodontida: Colepidae)

Ji Hye Moon,

Ji Hye Kim,

Jae-Ho Jung

Brackish water populations of Pinacocoleps pulcher were collected from a lagoon in Korea. This species has never been described using silver impregnation and nuclear small subunit ribosomal RNA (SSU rRNA) gene. In the present study, we investigated the morphological and molecular attributes of P. pulcher using standard methods. The morphology was studied based on observations of live materials, silver-impregnated preparations, and scanning electron microscopy. The morphological characteristics are as follows: body size 80–90 × 40–50 µm in vivo, shape broadly ellipsoidal, body cross-section ellipsoidal, about seven anterior spines and about seven posterior spines, approximately 21 somatic ciliary rows, one macronucleus and one micronucleus, and a single caudal cilium. The SSU rRNA gene tree supports a sister relationship of P. pulcher to the genus Apocoleps, not P. tesselatus.
Słowa kluczowe: ciliate, redescription, taxonomy, SSU rDNA sequences, brackish water, Korea

Bock K. J. (1952) Über einige holo- und spirotriche Ciliaten aus den marinen Sandgebieten der Kieler Bucht. Zool. Anz. 149: 107–115

Borror A. C. (1963) Morphology and ecology of the benthic ciliated protozoa of Alligator Harbor, Florida. Arch. Protistenk. Bd. 106: 465–534

Chen X., Warren A., Song W. (2009) Taxonomic studies on a new marine ciliate, Apocoleps magnus gen. nov., spec. nov. (Ciliophora, Colepidae), isolated from Qingdao, China. J. Ocean Univ. China 8: 317–321

Chen X., Wang Y., Long H., Al-Rasheid, K. A. S., Warren A., Song W. (2010) Mphological studies on two marine colepid ciliates from Qingdao, China, Nolandia orientalis spec. nov. and Pinacocoleps similis (Kahl, 1933) comb. nov. (Ciliophora, Colepidae). Eur. J. Protistol. 46: 254–262

Chen X., Shazib S. U. A., Kim J. H., Jang S. W., Shin M. K. (2016). Morphological description and molecular phylogeny of two species of Levicoleps (Ciliophora, Prostomatida), L. taehwae nov. spec. and L. biwae jejuensis nov. subspec., collected in Korea. J. Eukaryot. Microbiol. 63: 471–480

Darriba D., Taboada G. L., Doallo R., Posada D. (2012) jModel
Test2: more models, new heuristics and parallel computing. 
Nat. Methods 9: 772

Diesing K. M. (1865) Revision der Prothelminthen. Abtheilung: Amastigen. I. Amastigen ohne Peristom. Sber. Akad. Wiss. Wien. 52: 505–579

Foissner W. (2014) An update of ‘basic light and scanning electron microscopic methods for taxonomic studies of ciliate protozoa’ Int. J. Syst. Evol. Microbiol. 64: 271–292

Foissner W., Kusuoka Y., Shimano S. (2008) Morphology and gene sequence of Levicoleps biwae n. gen., n. sp. (Ciliophora, Prostomatida), a proposed endemic from the ancient Lake Biwa, Japan. J. Eukaryot. Microbiol. 55: 185–200

Hartwig E. (1973) Die Ciliaten des Gezeiten-Sandstrandes der Nordseeinsel Sylt. I. Systematik. Abh. math-naturw. Kl. Akad. Wiss. Mainz. Mikrofauna Meer. 18: 1–69

Kahl A. (1930) Urtiere oder Protozoa I: Wimpertiere order Ciliata (Infusoria) 1. Allegemeiner Teil und Prostomata. Tierwelt Dtl. 18: 1–180

Kahl A. (1933) Ciliata Libera et Ectocommensalia. Tierwelt Nord- und Ostsee 23 (Teil II, c3): 29–146

Kearse, M., Moir, R., Wilson, A., Stones-Havas, S., Cheung, M., Sturrock, S., Buxton, S., Cooper, A., Markowitz, S., Duran, C., Thierer, T., Ashton, B., Mentjies, P., Drummond, A. (2012) Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics 28: 1647–1649.

Lu B., Huang J., Chen X. (2013) The morphology and SSU rRNA gene sequence analysis of a poorly-known brackish water ciliate, Pinacocoleps tesselatus (Kahl, 1930) (Ciliophora, Colepidae) from Hangzhou Bay, China. Zootaxa 3637: 123–130

Lu B., Ma M., Gao F., Shi Y., Chen X. (2016) Morphology and molecular phylogeny of two colepid species from China, Coleps amphacanthus Ehrenberg, 1833 and Levicoleps biwae jejuensis Chen et al., 2016 (Ciliophora, Prostomatida). Zoological Research 37: 176–185

Lynn D. H. (2008) The ciliated Protozoa: characterization, classification, and guide to the literature, 3rd edn. Springer, Dordrecht

Nguyen L., Schmidt H. A., Haeseler A., Minh B. Q. (2015) IQ-TREE: a fast and effective stochastic algorithm for estimating maximum likelihood phylogenies. Mol. Biol. Evol. 32: 268–274

Noland L. E. (1937) Observations on marine ciliates of the Gulf Coast of Florida. Trans. Am. Micros. Soc. 56: 160–171

Pan X., Bourland W. A., Song W. (2013) Protargol Synthesis: An In-house Protocol. J. Eukaryot. Microbiol. 60: 609–614

Ronquist F., Teslenko M., van der Mark P., Ayres D. L., Darling A., Hohna S., Larget B., Liu L., Suchard M. A., Huelsenbeck J. P. (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Syst. Biol. 61: 539–542

Spiegel A. (1926) Einige neue marine Ciliaten. Arch. Protistenkd. 55: 184–190

Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol. Biol. Evol. 28: 2731–2739

Czasopismo w darmowym dostępie.

Czasopismo ukazuje się w sposób ciągły on-line.
Pierwotną formą czasopisma jest wersja elektroniczna.