Spore dimorphism in Nosema pyrausta (Microsporidia, Nosematidae): from morphological evidence to molecular genetic verification

I. V Grushevaya,

A. N. Ignatieva,

S. M. Malysh,

I. V. Senderskiy,

I. V. Zubarev,

A. G. Kononchuk


Microsporidia infection rate in Ostrinia nubilalis larvae collected in Russia in 2011-2016 ranged from 0 to 16 %. Totally, among 262 examined insects, there were as many as 13 infected specimens, resulting in average prevalence of 5 % over the period indicated. In all positive samples but one diplokaryotic spores 4 um long were observed corresponding to diagnosis of Nosema pyrausta. Nevertheless, in one case (i.e. 0.4 %) the infected larva contained monokaryotic spores about 2 um long. After experimental infection of a substitute host Ostrinia furnacalis with monokaryotic spores only Nosema-like spores were observed in laboratory assays. Ribosomal RNA and RPB1 gene portions were 100 % identical in samples of both mono- and diplokaryotic spores. This observation shows that Nosema pyrausta can form uninucleate spores under yet to be described conditions in nature and that molecular genetic analysis is essential for correct species identification. 

Słowa kluczowe: microsporidia, corn borer, life cycle, molecular genetic identification

Becnel J. J., Andreadis T. G. (1999) Microsporidia in insects. In: Wittner M., Weiss L. M., (eds.), The Microsporidia and microsporidiosis, American Society Microbiology Press, Washington DC. 447–501

Franz J. M., Huger A. M. (1971) Microsporidia causing the collapse of an outbreak of the green tortrix Tortrix viridana L. in Germany. Proс. Internat. Colloq. Insect Pathol. 4th College Park, MD. 48–53

Hill R. E., Gary W. J. (1979) Effects of the microsporidium, Nosema pyrausta, on field populations of European corn borers in Nebraska. Environ. Entomol. 8: 91–95

Issi I. V. (1986) Microsporidia as a phylum of parasitic protozoa. Protozoologiya. (Leningrad) 10: 1–136 (in Russian)

Kyei-Poku G., Sokolova Y. Y. (2017) The microsporidium Nosema disstriae (Thomson 1959): Fine structure and phylogenetic position within the N. bombycis clade. J. Invertebr. Pathol. 143: 90–103; https://doi.org/10.1016/j.jip.2016.12.003

Lewis L. C., Sumerford D. V., Bing L. A., Gunnarson R. D. (2006) Dynamics of Nosema pyrausta in natural populations of the European corn borer, Ostrinia nubilalis: A six-year study. Biocont. 51: 627–642

Lipa J. J. (1976) Microsporidians parasitizing the green tortrix in Poland and their role in the collapse of the tortrix outbreak in Puszcza Niepolomicka during 1970–1974. Acta Protozool. 15: 529–536

Lipa J. J. (1977) Thelohania ostriniae n.sp., a new microsporidian parasite of the European corn borer Ostrinia nubilalis Hnb. (Lepidoptera, Pyralidae). Acta Protozool. 16: 151–155

Malysh Yu. M., Tokarev Yu. S., Sitnikova N. V., Kononchuk A. G., Gruschetskaya T. A., Frolov A. N. (2011) Incidence of microsporidian infection of stem borers of the genus Ostrinia (Lepidoptera: Crambidae) in the Krasnodar Territory. Parazitologiya. 45: 234–243 (in Russian)

Paillot A. (1927) Sur deux protozoaires nouveaux parasites des chenilles de Pyrausta nubilalis Hubner. Compt. Rend. l’Acad. Sci. Paris 185: 673–675

Sambrook J., Fritsch E., Maniatis T. (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York

Solter L. F., Hajek A. E. (2009) Control of gypsy moth, Lymantria dispar, in North America since 1878. Use of microbes for control and eradication of invasive arthropods. Springer Netherlands. 181–212

Tokarev Yu. S., Malysh J. M., Kononchuk A. G., Seliverstova E. V., Frolov A. N., Issi I. V. (2015) Redefinition of Nosema pyrausta (Perezia pyraustae Paillot 1927) based upon ultrastructural and molecular phylogenetic studies. Parasitol. Res. 114: 759–761; https://doi.org/10.1007/s00436-014-4272-3

van Frankenhuyzen K., Ryall K., Liu Y., Meating J., Bolan P., Scarr T. (2011) Prevalence of Nosema sp. (Microsporidia: Nosematidae) during an outbreak of the jack pine budworm in Ontario. J. Invertebr. Pathol. 108: 201–208; https://doi.org/10.1016/j.jip.2011.09.002

Weiss L. M., Vossbrinck C. R. (1999) Molecular biology, molecular phylogeny, and molecular diagnostic approaches to the Microsporidia. In: Wittner M., Weiss L. M. (eds.), The Microsporidia and microsporidiosis. American Society Microbiology Press, Washington DC. 129–171

Wilson G. G. (1973) Incidence of microsporidia in a field population of a spruce budworm. Bimonth. Res. Can. For Serv. 29: 35–60

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