Phenotypic and Genotypic Characterization of Eimeria caviae from Guinea Pigs (Cavia porcellus)

Gilberto Flausino,

Carlos W. G. Lopes,

Walter L. Teixeira-Fillho,

Tassia T. Furtado,

Douglas Mcintosh,

Bruno P. Berto

Abstrakt

Coccidiosis in Guinea pigs (Cavia porcellus) has been frequently associated with the presence of Eimeria caviae; however, this coccidium has never been characterized in detail. This study aimed to present the phenotypic and genotypic characterization of E. caviae from guinea pigs reared under rustic breeding conditions in Brazil. Eimeria caviae oocysts are polymorphic, being sub-spherical, ovoidal or ellipsoidal, 20.9 × 17.7 µm, with a smooth or slightly rough and bi-layered wall, ~ 1.0 µm. Micropyle and oocyst residuum are absent, but one polar granule is present. Sporocysts are ellipsoidal, 10.8 × 6.4 µm. Stieda and parastieda bodies are present, sporocyst residuum is present and sporozoites posses a refractile body and a nucleus. Linear regressions and histograms were performed and confirmed the polymorphism of the oocysts. The internal transcribed spacer 1 of the ribosomal RNA gene (ITS-1 rRNA) of the isolates was sequenced and showed no significant similarity to the orthologous region of other Eimeria species.

Słowa kluczowe: oocysts, coccidiosis, diagnostic, morphology, morphometry, ITS-1, Rio de Janeiro, Brazil
References

Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. (1990) Basic local alignment search tool. J. Mol. Biol. 215: 403–410

Andrade A., Pinto S., Oliveira R. (2002) Animais de Laboratório: Criação e experimentação. Fiocruz, Rio de Janeiro

Berto B. P, Lopes B. do B., Flausino W., Teixeira-Filho W. L., Lopes C. W. G. (2008a) Contribution on the study of Isospora hemidactyli Carini, 1936 and a report of an adeleid pseudoparasite of the house gecko Hemidactylus mabouia, from the Rio de Janeiro Metropolitan Region, Brazil. Rev. Bras. Parasitol. Vet. 17: 150–154

Berto B. P., Flausino W., Almeida C. R. R., Lopes C. W. G. (2008b) Polymorphism of Tyzzeria parvula (Kotlán, 1933) Klimes, 1963 (Apicomplexa: Eimeriidae) oocysts from the greylag geese Anser anser L., 1758 conditioned in two distinct sites. Rev. Bras. Med. Vet. 30: 215–219

Berto B. P., Cardozo S. V., Teixeira-Filho W. L., Ferreira A. M. R., Lopes C. W. G. (2008c) Aflatoxin effect on the oocysts morphometry and contribution on the morphology of Eimeria bateri Bhatia, Pandey and Pande, 1965 of the Japanese quail Coturnix japonica, in Brazil. Rev. Bras. Parasitol. Vet. 17: 235–238

Berto B. P., Luz H., Flausino W., Teixeira-Filho W. L., Ferreira I., Lopes C. W. G. (2011) Isosporoid Coccidia (Apicomplexa: Eimeriidae) parasites of tanagers (Passeriformes: Thraupidae) from the Marambaia Island, Brazil. Pesq. Vet. Bras. 31: 798–805

Catchpole J., Norton C., Joyner L. (1975) The occurrence of Eimeria weybridgensis and other species of coccidia in lambs in England and Wales. Br. Vet. J. 131: 392–401

Duszynski D. (1971) Increase in size of Eimeria separata oocysts during patency. J. Parasitol. 57: 948–952

Duszynski D., Wilber P. (1997) A guideline for the preparation of species descriptions in the Eimeridae. J. Parasitol. 83: 333–336

Fayer R. (1980) Epidemiology of protozooan infection: the Coccidia. Vet. Parasitol6: 75–103

Fujino T., Matsui T., Morii T. (1991) Endogenous stages and the other biological characteristics of Eimeria caviae in Guinea pigs. Kisechugaku Zasshi. 40: 368–373

Gardner S., Duszynski D. (1990) Polymorphism of eimerian oocysts can be a problem in naturally infected hosts: an example from subterranean rodents in Bolivia. J. Parasitol76: 805–811

Gomez F., Navarrete I., Rodriguez R. (1982) Influencia de los factores ambientales sobre diferentes poblaciones de Isospora lacazei Labbé, 1983 (Protozoa: Apicomplexa). Rev. Iber. Parasitol42: 185–196

Hankinson G., Murphy J., Fox J. (1982) Diagnostic exercise. Eimeria caviae infection with concurrent Balantidium coli infection. Lab. Anim. Sci32: 35–36

Henry D. (1932) Coccidiosis of the guinea pigs. Univ. Calif. Publ. Zool. 37: 211–268

Joyner L. (1982) Host and Site specificity. In: Long P., The biology of the Coccidia. University Park Press, Baltimore, 35–62

Kawahara F., Zhang G., Mingala C. N., Tamura Y., Koiwa M., Onuma M., Nunoya T. (2010) Genetic analysis and development of species-specific PCR assays based on ITS-1 region of rRNA in bovine Eimeria parasites. Vet. Parasitol174: 49–57

Lapage G. (1940) The study of coccidiosis in the Guinea pig. Vet. J. 96: 144–154, 190–202, 242–254, 280–295

Matsui T., Fujino T., Kobayashi F., Tsutsumi Y., Tsuji M. (1996) Attenuation of Eimeria caviae by selection for precocious development. Int. J. Parasitol. 26: 1243–1248

Moore C. (1976) A cytological study of the sporozoites of Eimeria caviae, a coccidian parasite of the domestic Guinea pig, Cavia porcellus. Thesis. Drake University, School of Graduate Studies. United States

Motriuk-Smith D., Seville R. S., Quealy L. L., Clinton E., Oliver C. E. (2011) Comparison of the ITS1 and ITS2 rDNA in Emeria callospermophili (Apicomplexa: Eimeriidae) from sciurid rodents. J. Parasitol. 97: 305–310

Muto T., Sugisaki M., Yusa T., Noguchi Y. (1985) Studies on coccidiosis in guinea pigs. Clinico-pathological observations. Experim. Anim. 34: 23–29

Norton C. C., Joyner L. P. (1981) Eimeria acervulina and E. mivati: oocysts, life-cycle and ability to develop in the chiken embryo. Parasitology83: 269–279

Oliveira U. C., Fraga J. S., Licois D., Pakandl M., Gruber A. (2011) Development of molecular assays for the identification of the 11 Eimeria species of the domestic rabbit (Oryctolagus cuniculus). Vet. Parasitol176: 275–280

Parker B., Duszynski D. (1986) Polymorphism of eimerian oocysts: a dilemma posed by working with some naturally infected hosts. J. Parasitol. 72: 602–604

Ramirez L., Berto B. P., Teixeira-Filho W., Flausino W., Meireles G., Rodrigues J., Almeida C., Lopes C. W. G. (2009b) Eimeria bareillyi from the domestic water buffalo, Bubalus bubalis, in the State of Rio de Janeiro, Brazil. Rev. Bras. Med. Vet. 31: 261–264

Rigby C. (1976) Natural infections of Guinea pigs. Lab. Anim. 10: 119–142

Sachser N. (1998) Of domestic and wild Guinea pigs: Studies in sociophysiology, domestication, and social evolution. Naturwissen 85: 307–317

Sampaio I. B. M. (2002) Estatística aplicada à experimentação animal. FEP MVZ Editora, Belo Horizonte

Schnitzler B. E., Thebo P. L., Mattsson J. G., Tomley F. M., Shirley M. W. (1998) Development of a diagnostic PCR assay for the detection and discrimination of four pathogenic Eimeria species of the chicken. Avian Pathol. 27: 490–497

Schnitzler B. E., Thebo P. L., Tomley F. M., Uggla A., Shirley M. W. (1999) PCR identification of chicken Eimeria: A simplified read-out. Avian Pathol. 28: 89–93

Sheather A. (1924) Coccidiosis in the Guinea Pig. J. Comp. Path. 37: 243–246

Zwart P., Strik W. (1961) Further observations on Eimeria dolichotis, a coccidium of the Patagonian cavy (Dolichotis patagonica). J. Protozool8: 58–59

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